transgene相关论文
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目的 观察外源基因在猪血管内皮细胞及小鼠体内表达情况.方法 体外培养猪血管内皮细胞,脂质体转染法将pcDNA3-ICAM-2-Enhancer CD5......
目的 探讨黄精多糖对实验性阿尔茨海默病(AD)小鼠的疗效及作用机制.方法 26只淀粉样前体蛋白(amyloid precursor protein,APP)转基......
Breeding and Identification of Insect-Resistant Rice by Transferring Two Insecticidal Genes, sbk and
The plasmid of pCDMARUBA-Hyg, which contained two insect-resistance genes, sbk (modified from Cry1A(c)) and sck (modifie......
Preparation and Amplification of Colony of Goat Transgenic Fetal Fibroblast and Mammary Gland Epithe
[Objective] The aim was to explore technical system of making single transgenic positive cells become colony cells by am......
PiggyBac transposon has demonstrated its long-term and stable transposition on genomes of various species but lacking of......
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为探究吕家坨井田地质构造格局,根据钻孔勘探资料,采用分形理论和趋势面分析方法,研究了井田7......
目的将四倍体胚胎补偿技术与转基因相结合,构建基因打靶载体转化的胚胎干细胞(ESCs),获得小概率的同源重组事件,进而直接得到所要......
为探究吕家坨井田地质构造格局,根据钻孔勘探资料,采用分形理论和趋势面分析方法,研究了井田7......
为探究吕家坨井田地质构造格局,根据钻孔勘探资料,采用分形理论和趋势面分析方法,研究了井田7......
Involvement of transcriptional enhancers in the regulation of developmental expression of yellow gen
为探究吕家坨井田地质构造格局,根据钻孔勘探资料,采用分形理论和趋势面分析方法,研究了井田7......
Establishment of multiple shoot clumps from maize(Zea mays L.) and regeneration of herbicide-resista
为探究吕家坨井田地质构造格局,根据钻孔勘探资料,采用分形理论和趋势面分析方法,研究了井田7......
为探究吕家坨井田地质构造格局,根据钻孔勘探资料,采用分形理论和趋势面分析方法,研究了井田7......
Establishment and application of both FLP and Cre site-specific recombination systems at the same po
为探究吕家坨井田地质构造格局,根据钻孔勘探资料,采用分形理论和趋势面分析方法,研究了井田7......
Efficient and stable generation of transgenic silkworm, Bombyx mori with the lepidopteran derived tr
为探究吕家坨井田地质构造格局,根据钻孔勘探资料,采用分形理论和趋势面分析方法,研究了井田7......
为探究吕家坨井田地质构造格局,根据钻孔勘探资料,采用分形理论和趋势面分析方法,研究了井田7......
Robust and regulatory expression of defensin A gene driven by vitellogenin promoter in transgenic An
为探究吕家坨井田地质构造格局,根据钻孔勘探资料,采用分形理论和趋势面分析方法,研究了井田7......
[目的]为生产转基因克隆猪奠定基础.[方法]以反转录病毒转染的带有红色荧光蛋白(RFP)基因的猪胎儿成纤维细胞作为核移植的核供体,......
Establishment of multiple shoot clumps from maize(Zea mays L.) and regeneration of herbicide-resista
A kind of quick, efficient and season-free inducing embryoid and multiple shoot clumps system from shoot tip meristems t......
Focusing on several commonly used insect resistance genes,we reviewed the advances in insect-resistant transgenic rice,a......
Purpose: To investigate the results of the interferon-gamma(IFN-y) gene transfer and transient expression in human Tenon......
The gonad is an essential organ for generating sperm and ova in vertebrates. This review describes several pilot studies......
由于植物分子育种技术克服了常规育种方法周期长、预见性差、选择效率低的局限性 ,可以打破物种界限 ,实现优良基因重组和聚合,能......
Mdm2 and Mdm4 are two key negative regulators of the tumor suppressor p53.Deletion of either Mdm2 or Mdm4 induces p53-de......
以FAM和BHQ1两种荧光素分别标记为报告染料和淬灭染料,建立了转基因发酵豆制品中抗草甘膦转基因EPSPS、大豆凝集素Lectin基因的Taq......
Involvement of transcriptional enhancers in the regulation of developmental expression of yellow gen
Upstream regulatory region and flanking DNA of yellow gene wereisolated and cloned from a Drosophila genomic library. A ......
Human lysozyme is a 130-aa (amino acid) alkaline polypeptide, and has both anti-bacterial and anti-viral properties whic......
The antisense Caffeoyl CoA O-methyltransferase (CCoAOMT) cDNA was transformed into Chinese white poplar (Populus tomento......
Robust and regulatory expression of defensin A gene driven by vitellogenin promoter in transgenic An
The use of genetically modified mosquitoes to reduce or replace field populations is a new strategy to control mosquito-......
Efficient and stable generation of transgenic silkworm, Bombyx mori with the lepidopteran derived tr
The piggyBac transposable element was successfully used for generation of the transgenic silkworm, Bombyx mori. The EGFP......
A mutagenesis library was constructed using GPS-LS system to insert a random 5 aa into the 5-enolpyruvylshikimate-3-phos......
目的 探讨慢病毒介导RNA干扰(RNAi)抑制APP695基因表达对体外培养的APP695转基因小鼠皮质神经元细胞分泌β淀粉样蛋白(Aβ)的影响.......
转座子是一种不依赖于同源性重组即可在宿主基因组中发生基因座位置改变的DNA片段.PiggyBac(PB)转座子是一种可移动的遗传元素,并......
Viruses are very widespread in nature,and they cause severe diseases and yield losses in potato production.The transfer ......
以冬小麦品种8901、5-98、99-92和104等品种的幼穗和幼胚为材料,用基因枪转化含逆境诱导转录因子DREB和bar基因的质粒pBAC128F(7 0......
以转 GhABF2转录因子马铃薯试管苗为材料，进行苗期耐盐性研究，以期了解该作物对逆境的适应性。实验结果表明：在不同浓度NaCl胁迫条件......
ed in plant stress response and that OsDHODH1 could be used in engineering crop plants with enhanced tolerance to salt a......
旨在构建可高效表达 pGH 基因和 IGF-Ⅰ基因的双基因共表达载体，制备转双基因（pGH ＋IGF-Ⅰ）猪，以期探索 pGH 基因和 IGF-Ⅰ基因对猪生长......
目的 建立一种可连续定量监测的原位前列腺癌动物模型.方法 利用分子克隆技术构建表达荧光素酶的慢病毒载体,用慢病毒将GFP-Lucife......
Introgression of transgenic crop alleles: Its evolutionary impacts on conserving genetic diversity o
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The full length osRACD cDNA sequence was subcloned into the pBI121 plasmid in the antisense orientation under the contro......
文章对建立转基因动物的方法--显微注射法、逆转录病毒感染法、胚胎干细胞介导法、精子载体法和体细胞核移植法作了系统阐述,指出......
为了建立有效地用于转基因动物生产的慢病毒表达系统,以线虫C.briggsae的ω-3脂肪酸去饱和酶基因（sFat-1）为目的基因构建了慢病毒表......
目的:建立人BAFF转基因斑马鱼模型,探讨其在自身免疫性疾病发病中的作用.方法:RT-PCR法由人淋巴瘤细胞克隆了人BAFF基因全长855 bp......
为了研究转基因香石竹品系月之霓裳Moonshade、月之伊人Moonlite及其亲本品种FE123在农艺性状方面是否存在差异,提供转基因香石竹......
